D-Ala-D-Ala ligase - ARO:3003970-AAN62561.1
Model based on:
Entry features
| GENE | D-Ala-D-Ala ligase | |
|---|---|---|
| DESCRIPTION | D-Ala-D-Ala ligase | |
| ORGANISM | Enterococcus gallinarum | |
| TAXONOMY | Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;Enterococcus. | |
| UNIPROT | Q8GJG5 | |
| NCBI | AAN62561.1 | |
| ARO term | ARO:3003970 | |
| Template | 3k3p A | |
| RESISTANCE | glycopeptide |
Cross-references
| REFSEQ | WP_003128558.1; NZ_NSCY01000006.1 | |
|---|---|---|
| PFAM | PF01820 - Dala_Dala_lig_N; PF07478 - Dala_Dala_lig_C | |
| PATRIC | fig|1353.11.peg.1165 | |
| EMBL | AY168442; AAN62561.1; AY971750; AAX89143.1; AY971751; AAX89144.1; JXKP01000002; OJG50829.1; NSCY01000006; PCD93563.1 | |
| EC | 6.3.2.4 | |
| Catalytic activity | ATP + 2 D-alanine = ADP + phosphate + D- alanyl-D-alanine; ECO:0000256| HAMAP-Rule:MF_00047 ; ECO:0000256| SAAS:SAAS00910566 | |
| PATHWAY | Cell wall biogenesis; peptidoglycan biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00047, ECO:0000256|SAAS:SAAS00910582}. |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0005524 | F:ATP binding | IEA | |
| GO:0008716 | F:D-alanine-D-alanine ligase activity | IEA | |
| GO:0046872 | F:metal ion binding | IEA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0008360 | P:regulation of cell shape | IEA | |
| GO:0009252 | P:peptidoglycan biosynthetic process | IEA | |
| GO:0071555 | P:cell wall organization | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005737 | C:cytoplasm | IEA |
Mutation panel
| Mutation | Phenotype | Reference | Map mutation on Model |
|---|---|---|---|
| D295G | resistant | PMID:11274474 | 295 |
| E13G | resistant | PMID:11274474 | 13 |
| G99R | resistant | PMID:11274474 | 99 |
| P313L | resistant | PMID:11274474 | 313 |
| V241D | resistant | PMID:11274474 | 241 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| D295G | 302 |
| E13G | 13 |
| G99R | 106 |
| P313L | 321 |
| V241D | 248 |