########################################
# Program: needle
# Rundate: Tue 12 Jun 2018 19:28:32
# Commandline: needle
#    -outfile /Users/teresatavella/Documents/negativedb/seqid30_85/sp_P00956_SYI_ECOLI_1ffy_A/needle.txt
#    -asequence /Users/teresatavella/Documents/negativedb/seqid30_85/sp_P00956_SYI_ECOLI_1ffy_A/sp_P00956_SYI_ECOLI_Isoleucine--tRNA_ligase_OS_Escherichia_coli_strain_K12_OX_83333_GN_ileS_PE_1_SV_5.fa
#    -bsequence /Users/teresatavella/Documents/negativedb/seqid30_85/sp_P00956_SYI_ECOLI_1ffy_A/1ffy_A.fa
#    -gapopen 10
#    -gapextend 0.5
# Align_format: srspair
# Report_file: /Users/teresatavella/Documents/negativedb/seqid30_85/sp_P00956_SYI_ECOLI_1ffy_A/needle.txt
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: sp_P00956_SYI_ECOLI_Isoleucine--tRNA_ligase_OS_Escherichia_coli_strain_K12_OX_83333_GN_ileS_PE_1_SV_5
# 2: 1ffy_A_mol_protein_length_917__ISOLEUCYL-TRNA_SYNTHETASE
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 967
# Identity:     404/967 (41.8%)
# Similarity:   579/967 (59.9%)
# Gaps:          79/967 ( 8.2%)
# Score: 1987.0
# 
#
#=======================================

sp_P00956_SYI      1 MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAAKKGKKT     50
                      .||:.||.:|:|.|||||.|..:||.:..:|..:|.|.......||.:|
1ffy_A_mol_pr      1 -MDYEKTLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNET     49

sp_P00956_SYI     51 FILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGL    100
                     ||||||||||||::|:||::||||||.||:.|.:.|:.:|||||||.|||
1ffy_A_mol_pr     50 FILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGL     99

sp_P00956_SYI    101 PIELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDW    150
                     |||..:.:: |...:|.:.||||.||:|:|..|::.|:|||.||||.||:
1ffy_A_mol_pr    100 PIEQALTKK-GVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDF    148

sp_P00956_SYI    151 SHPYLTMDFKTEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEV    200
                     :.||:|:..:.||..||..|::...|.::||.|||:|.....|:|||||:
1ffy_A_mol_pr    149 NDPYITLKPEYEAAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEI    198

sp_P00956_SYI    201 EYYDKTSPSIDVAFQAVDQDALKAKFAVSNVNGPISLVIWTTTPWTLPAN    250
                     ||:||.|.||.|||...|...:        |:.....:||||||||:|:|
1ffy_A_mol_pr    199 EYHDKRSASIYVAFNVKDDKGV--------VDADAKFIIWTTTPWTIPSN    240

sp_P00956_SYI    251 RAISIAPDFDYALVQIDGQAVILAKDLVESVMQRIG--------VTDYTI    292
                     .||::.|:..|....::|:..|:|:.|.::|.:.:.        ..:|| 
1ffy_A_mol_pr    241 VAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYT-    289

sp_P00956_SYI    293 LGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHTAPGHGPDD    342
                          |.|||.:...|||:..:...|.|||||.|||||.||||||||.||
1ffy_A_mol_pr    290 -----GKELEWVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD    334

sp_P00956_SYI    343 YVIGQKYGLETANPVGPDGTYLP--GTYPTLDGVNVFKANDIVVALLQEK    390
                     |::||:|.|...:|:...|.:..  |.:   :|:...|||..|..||.||
1ffy_A_mol_pr    335 YIVGQQYELPVISPIDDKGVFTEEGGQF---EGMFYDKANKAVTDLLTEK    381

sp_P00956_SYI    391 GALLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKG    440
                     ||||.::.:.||||..||.|.|:|||||||||.|:.:  :|...|..|:.
1ffy_A_mol_pr    382 GALLKLDFITHSYPHDWRTKKPVIFRATPQWFASISK--VRQDILDAIEN    429

sp_P00956_SYI    441 VQWIPDWGQARIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTL    490
                     ..:..:||:.||.:||.:|.:|.|||||.||||:.:|..::.|.:  .|.
1ffy_A_mol_pr    430 TNFKVNWGKTRIYNMVRDRGEWVISRQRVWGVPLPVFYAENGEII--MTK    477

sp_P00956_SYI    491 ELMEEVAKRVEVDGIQAWWDLDAKEIL-------GDEADQYVKVPDTLDV    533
                     |.:..||......|...|::.:||::|       |.....:.|..|.:||
1ffy_A_mol_pr    478 ETVNHVADLFAEHGSNIWFEREAKDLLPEGFTHPGSPNGTFTKETDIMDV    527

sp_P00956_SYI    534 WFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQHRGWFMSSLMISTAMKGK    583
                     ||||||:|..|::.|||.: ..||||||||||:||||.||:..|.|.:|.
1ffy_A_mol_pr    528 WFDSGSSHRGVLETRPELS-FPADMYLEGSDQYRGWFNSSITTSVATRGV    576

sp_P00956_SYI    584 APYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADILRLWVASTD    633
                     :||:.:|:|||.:||:|:|||||:||.:.|..|:.:.||||.||||:|||
1ffy_A_mol_pr    577 SPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSSTD    626

sp_P00956_SYI    634 YTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMVV    683
                     |..::.:||||||:.:|.||:||||.||:|.|:|.|:|..|.:...|::.
1ffy_A_mol_pr    627 YLADVRISDEILKQTSDDYRKIRNTLRFMLGNINDFNPDTDSIPESELLE    676

sp_P00956_SYI    684 LDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKD    733
                     :||:.:...:......:..||.:|:..:.|.:..|.:||:.:||||..||
1ffy_A_mol_pr    677 VDRYLLNRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKD    726

sp_P00956_SYI    734 RQYTAKADSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGERE    783
                     ..|..:.||..|||.||.||.|...:.:.:||||..||:|||.:.|..:|
1ffy_A_mol_pr    727 ILYIEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKE    776

sp_P00956_SYI    784 KYVFTGEWYEGLFGLAD-------SEAMNDAFWDELLKVRGEVNKVIEQA    826
                     :.|.          |||       .:|:.|. |...:.:|.:||:.:|.|
1ffy_A_mol_pr    777 ESVH----------LADMPKVVEVDQALLDK-WRTFMNLRDDVNRALETA    815

sp_P00956_SYI    827 RADKKVGGSLEAAVTLYAEPELSAK--LTALGDELRFVLLTSGATVADYN    874
                     |.:|.:|.||||.||:.:..:.:|.  ||:. |.|..:.:.|...|.|..
1ffy_A_mol_pr    816 RNEKVIGKSLEAKVTIASNDKFNASEFLTSF-DALHQLFIVSQVKVVDKL    864

sp_P00956_SYI    875 DAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKVAEHAEICGRC    924
                     |..|.|.:..     .:.:..|:||||.|||:|::|:|.|.|...:|.||
1ffy_A_mol_pr    865 DDQATAYEHG-----DIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRC    909

sp_P00956_SYI    925 ---VSNVAGDGEKRKFA    938
                        |.::.         
1ffy_A_mol_pr    910 QQVVKSLV---------    917


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