thyA - ARO:3004153-NP_217280.1
Structure
Entry features
| GENE | thyA |
|---|---|
| ORGANISM | Mycobacterium tuberculosis H37Rv |
| TAXONOMY | Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;Mycobacterium; Mycobacterium tuberculosis complex. |
| UNIPROT | P9WFR9 |
| NCBI | NP_217280.1 |
| ARO term | ARO:3004153 |
| RESISTANCE | aminosalicylate; para-aminosalicylic acid |
Cross-references
| STRING | 83332.Rv2764c | |
|---|---|---|
| REFSEQ | NP_217280.1; NC_000962.3; WP_003414075.1 | |
| PFAM | PF00303 - Thymidylat_synt | |
| PATRIC | fig|83332.111.peg.3075 | |
| KEGG | mtu:Rv2764c; mtv:RVBD_2764c | |
| EMBL | AL123456; CCP45563.1 | |
| BioCyc/EcoCyc/MetaCyc | MetaCyc:G185E-7013-MONOMER | |
| EC | 2.1.1.45 | |
| CHEMBL | CHEMBL1795162 | |
| Catalytic activity | 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP; ECO:0000255| HAMAP-Rule:MF_00008 ; ECO:0000269| PubMed:18493582 | |
| Active site | 146 146 Nucleophile | |
| PATHWAY | Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00008}. | |
| NUCLEOTIDE BINDING | 126 127 dUMP; shared with dimeric partner; 166 169 dUMP; 207 209 dUMP |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0004799 | F:thymidylate synthase activity | IDA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0006231 | P:dTMP biosynthetic process | IDA | |
| GO:0006235 | P:dTTP biosynthetic process | IEA | |
| GO:0046079 | P:dUMP catabolic process | IDA | |
| GO:0046677 | P:response to antibiotic | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005737 | C:cytoplasm | IEA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| C161T | resistant | PMID:25421465 |
| F152V | resistant | PMID:25421465 |
| G91R | resistant | PMID:25421465 |
| H207R | resistant | PMID:25421465 |
| H75N | resistant | PMID:25421465 |
| I211V | resistant | PMID:25421465 |
| P224L | resistant | PMID:25421465 |
| R126Q | resistant | PMID:25421465 |
| R235P | resistant | PMID:25421465 |
| S105P | resistant | PMID:25421465 |
| T202A | resistant | PMID:25421465 |
| T22A | resistant | PMID:25421465 |
| V77F | resistant | PMID:25421465 |
| W101R | resistant | PMID:25421465 |
| W83C | resistant | PMID:25421465 |
| Y36C | resistant | PMID:25421465 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| C161T | 171 |
| F152V | 162 |
| G91R | 101 |
| H207R | 217 |
| H75N | 85 |
| I211V | 221 |
| P224L | 234 |
| R126Q | 136 |
| R235P | 245 |
| S105P | 115 |
| T202A | 212 |
| T22A | 32 |
| V77F | 87 |
| W101R | 111 |
| W83C | 93 |
| Y36C | 46 |