nfsA - ARO:3003751-NP_415372.1
Structure
Entry features
| GENE | nfsA | |
|---|---|---|
| DESCRIPTION | Modulator of drug activity A | |
| ORGANISM | Escherichia coli str. K-12 substr. MG1655 | |
| TAXONOMY | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;Enterobacteriaceae; Escherichia. | |
| UNIPROT | P17117 | |
| NCBI | NP_415372.1 | |
| ARO term | ARO:3003751 | |
| RESISTANCE | nitrofuran; nitrofurantoin |
Cross-references
| STRING | 316385.ECDH10B_0921 | |
|---|---|---|
| REFSEQ | NP_415372.1; NC_000913.3; WP_000189159.1; NZ_LN832404.1 | |
| PFAM | PF00881 - Nitroreductase | |
| PATRIC | fig|1411691.4.peg.1427 | |
| KEGG | ecj:JW0835; eco:b0851 | |
| EMBL | AP009048; BAA35562.1; D38308; BAA07425.1; U00096; AAC73938.1; U18655; AAC43450.1; X15859; CAA33867.1; ALT_FRAME | |
| BioCyc/EcoCyc/MetaCyc | EcoCyc:EG11261-MONOMER; MetaCyc:EG11261-MONOMER | |
| INTACT | P17117 | |
| EC | 1.-.-.- | |
| Drugbank | DB00698 - Nitrofurantoin; DB03247 - Riboflavin Monophosphate | |
| Catalytic activity | An oxidized nitroaromatic compound + NADPH = a reduced nitroaromatic compound + NADP(+); ECO:0000269| PubMed:8755878 | |
| NUCLEOTIDE BINDING | 11 15 FMN; 128 131 FMN; 167 169 FMN |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0010181 | F:FMN binding | IDA | |
| GO:0016657 | F:oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor | IDA | |
| GO:0034567 | F:chromate reductase activity | IDA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005829 | C:cytosol | IDA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| G131D | resistant | PMID:18544599 |
| R133S | resistant | PMID:26552976 |
| R15C | resistant | PMID:18544599 |
| R203C | resistant | PMID:18544599 |
| R203L | resistant | PMID:18544599 |
| S33R | resistant | PMID:18544599 |
| Y45C | resistant | PMID:18544599 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| G131D | 131 |
| R133S | 133 |
| R15C | 15 |
| R203C | 203 |
| R203L | 203 |
| S33R | 33 |
| Y45C | 45 |