tlyA - ARO:3003445-AAK46002.1
Structure
Entry features
| GENE | tlyA | |
|---|---|---|
| DESCRIPTION | 16S rRNA (cytidine1409-2'-O)-methyltransferase | |
| ORGANISM | Mycobacterium tuberculosis CDC1551 | |
| TAXONOMY | Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;Mycobacterium; Mycobacterium tuberculosis complex. | |
| UNIPROT | P9WJ62 | |
| NCBI | AAK46002.1 | |
| ARO term | ARO:3003445 | |
| RESISTANCE | aminoglycoside; streptomycin |
Cross-references
| REFSEQ | WP_003408382.1; NZ_KK341227.1 | |
|---|---|---|
| PFAM | PF01479 - S4; PF01728 - FtsJ | |
| PATRIC | fig|83331.31.peg.1861 | |
| KEGG | mtc:MT1733 | |
| EMBL | AE000516; AAK46002.1 | |
| EC | 2.1.1.226; 2.1.1.227 | |
| Catalytic activity | S-adenosyl-L-methionine + cytidine(1409) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1409) in 16S rRNA; ECO:0000250| UniProtKB:P9WJ63 S-adenosyl-L-methionine + cytidine(1920) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1920) in 23S rRNA; ECO:0000250| UniProtKB:P9WJ63 |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0003723 | F:RNA binding | IEA | |
| GO:0008168 | F:methyltransferase activity | IEA | |
| GO:0090729 | F:toxin activity | IEA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0006364 | P:rRNA processing | IEA | |
| GO:0044179 | P:hemolysis in other organism | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005576 | C:extracellular region | IEA | |
| GO:0005618 | C:cell wall | IEA | |
| GO:0005737 | C:cytoplasm | IEA | |
| GO:0016020 | C:membrane | IEA | |
| GO:0020002 | C:host cell plasma membrane | IEA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| A67E | resistant | PMID:15673735 |
| A91E | resistant | PMID:15673735 |
| E238K | resistant | PMID:15673735 |
| F185L | resistant | PMID:15673735 |
| K69E | resistant | PMID:15673735 |
| L118P | resistant | PMID:15673735 |
| L150P | resistant | PMID:15673735 |
| N236K | resistant | PMID:15673735 |
| P183L | resistant | PMID:15673735 |
| R14W | resistant | PMID:15673735 |
| V128E | resistant | PMID:15673735 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| A67E | 67 |
| A91E | 91 |
| E238K | 238 |
| F185L | 185 |
| K69E | 69 |
| L118P | 118 |
| L150P | 150 |
| N236K | 236 |
| P183L | 183 |
| R14W | 14 |
| V128E | 128 |