EF-Tu - ARO:3003368-AAA50993.1
Structure
Entry features
| GENE | EF-Tu | |
|---|---|---|
| DESCRIPTION | Bacteriophage Q beta RNA-directed RNA polymerase subunit III | |
| ORGANISM | Escherichia coli | |
| TAXONOMY | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;Enterobacteriaceae; Escherichia. | |
| UNIPROT | P0CE47 | |
| NCBI | AAA50993.1 | |
| ARO term | ARO:3003368 | |
| RESISTANCE | elfamycin; kirromycin-like antibiotics; rifampicin; pulvomycin |
Cross-references
| STRING | 316385.ECDH10B_3514 | |
|---|---|---|
| REFSEQ | NP_417798.1; NC_000913.3; WP_000031783.1; NZ_LN832404.1 | |
| PTM | ||
| PFAM | PF00009 - GTP_EFTU; PF03143 - GTP_EFTU_D3; PF03144 - GTP_EFTU_D2 | |
| PATRIC | fig|1411691.4.peg.3392 | |
| KEGG | ecj:JW3301; eco:b3339 | |
| EMBL | AF058450; AAC14286.1; AP009048; BAE77952.1; J01690; AAA50993.1; M10459; AAA24702.1; U00096; AAC76364.1; U18997; AAA58136.1 | |
| BioCyc/EcoCyc/MetaCyc | EcoCyc:EG11036-MONOMER | |
| INTACT | P0CE47 | |
| NUCLEOTIDE BINDING | 136 139 GTP; 19 26 GTP; 81 85 GTP |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0003746 | F:translation elongation factor activity | IEA | |
| GO:0003924 | F:GTPase activity | IEA | |
| GO:0005525 | F:GTP binding | IEA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0046677 | P:response to antibiotic | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005737 | C:cytoplasm | HDA | |
| GO:0005886 | C:plasma membrane | IEA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| A376S | resistant | PMID:12370016 |
| A376T | resistant | PMID:12370016 |
| A376V | resistant | PMID:12370016 |
| E379K | resistant | PMID:12370016 |
| G317D | resistant | PMID:12370016 |
| L121Q | resistant | PMID:12370016 |
| Q125E | resistant | PMID:12370016 |
| Q125K | resistant | PMID:7525272 |
| Q125R | resistant | PMID:12370016 |
| Q330H | resistant | PMID:12370016 |
| R231C | resistant | PMID:NA |
| R334C | resistant | PMID:NA |
| T335A | resistant | PMID:NA |
| Y161C | resistant | PMID:12370016 |
| Y161D | resistant | PMID:12370016 |
| Y161N | resistant | PMID:12370016 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| A376S | 376 |
| A376T | 376 |
| A376V | 376 |
| E379K | 379 |
| G317D | 317 |
| L121Q | 121 |
| Q125E | 125 |
| Q125K | 125 |
| Q125R | 125 |
| Q330H | 330 |
| R231C | 231 |
| R334C | 334 |
| T335A | 335 |
| Y161C | 161 |
| Y161D | 161 |
| Y161N | 161 |