parC - ARO:3003308-AAC76055.1
Structure
Entry features
| GENE | parC | |
|---|---|---|
| DESCRIPTION | Topoisomerase IV subunit A | |
| ORGANISM | Escherichia coli str. K-12 substr. MG1655 | |
| TAXONOMY | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;Enterobacteriaceae; Escherichia. | |
| UNIPROT | P0AFI2 | |
| NCBI | AAC76055.1 | |
| ARO term | ARO:3003308 | |
| RESISTANCE | ciprofloxacin; enoxacin; fluoroquinolone; gatifloxacin; grepafloxacin; levofloxacin; lomefloxacin; moxifloxacin; nalidixic acid; norfloxacin; ofloxacin; pefloxacin; sparfloxacin; trovafloxacin |
Cross-references
| STRING | 316385.ECDH10B_3193 | |
|---|---|---|
| REFSEQ | NP_417491.1; NC_000913.3; WP_001281881.1; NZ_LN832404.1 | |
| PFAM | PF00521 - DNA_topoisoIV; PF03989 - DNA_gyraseA_C | |
| PATRIC | fig|1411691.4.peg.3711 | |
| KEGG | ecj:JW2987; eco:b3019 | |
| EMBL | AP009048; BAE77075.1; L22025; AAC36840.1; M58408; AAA24297.1; ALT_FRAME; M63491; AAA24396.1; U00096; AAC76055.1; U28377; AAA69187.1 | |
| BioCyc/EcoCyc/MetaCyc | EcoCyc:EG10686-MONOMER | |
| INTACT | P0AFI2 | |
| EC | 5.99.1.3 | |
| Drugbank | DB00817 - Rosoxacin | |
| CHEMBL | CHEMBL1895 | |
| Catalytic activity | ATP-dependent breakage, passage and rejoining of double-stranded DNA; ECO:0000255| HAMAP-Rule:MF_00936 ; ECO:0000269| PubMed:12269820 ; ECO:0000269| PubMed:16023670 ; ECO:0000269| PubMed:21300644 | |
| Active site | 120 120 O-(5'-phospho-DNA)-tyrosine intermediate | |
| MINT | P0AFI2 |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0003677 | F:DNA binding | IDA | |
| GO:0003918 | F:DNA topoisomerase type II (ATP-hydrolyzing) activity | IEA | |
| GO:0005524 | F:ATP binding | IEA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0006265 | P:DNA topological change | IDA | |
| GO:0007059 | P:chromosome segregation | IDA | |
| GO:0007062 | P:sister chromatid cohesion | IMP | |
| GO:0030541 | P:plasmid partitioning | IDA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005694 | C:chromosome | IEA | |
| GO:0005737 | C:cytoplasm | IDA | |
| GO:0005829 | C:cytosol | IDA | |
| GO:0009330 | C:DNA topoisomerase complex (ATP-hydrolyzing) | IDA | |
| GO:0019897 | C:extrinsic component of plasma membrane | IDA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| E84G | resistant | PMID:25631675 |
| S80I | resistant | PMID:25631675 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| E84G | 91 |
| S80I | 87 |