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PVAR3D
gyrB - ARO:3003303-BAE77595.1
Structure
Entry features
GENE gyrB
DESCRIPTION Type IIA topoisomerase subunit GyrB
ORGANISM Escherichia coli str. K-12 substr. W3110
TAXONOMY Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;Enterobacteriaceae; Escherichia.
UNIPROT P0AES6
NCBI BAE77595.1
ARO term ARO:3003303
RESISTANCE aminocoumarin; clorobiocin; coumermycin A1; novobiocin; nalidixic acid and enoxacin; nalidixic acid; para-aminosalicylic acid
Cross-references
STRING 316407.85676345
REFSEQ WP_000072067.1; NZ_LN832404.1; YP_026241.1; NC_000913.3
PFAM PF00204 - DNA_gyraseB; PF00986 - DNA_gyraseB_C; PF01751 - Toprim; PF02518 - HATPase_c
PATRIC fig|511145.12.peg.3823
KEGG ecj:JW5625; eco:b3699
EMBL AP009048; BAE77595.1; D87842; BAA20341.1; L10328; AAA62050.1; M15548; AAA23949.1; U00096; AAT48201.1; X04341; CAA27871.1
BioCyc/EcoCyc/MetaCyc EcoCyc:EG10424-MONOMER; MetaCyc:EG10424-MONOMER
INTACT P0AES6
EC 5.99.1.3
Drugbank DB00817 - Rosoxacin; DB03966 - Clorobiocin; DB04395 - Phosphoaminophosphonic Acid-Adenylate Ester; DB05488 - 99mTc-ciprofloxacin
CHEMBL CHEMBL1826
Catalytic activity ATP-dependent breakage, passage and rejoining of double-stranded DNA; ECO:0000255| HAMAP-Rule:MF_01898 ; ECO:0000269| PubMed:12051842 ; ECO:0000269| PubMed:12051843 ; ECO:0000269| PubMed:18642932 ; ECO:0000269| PubMed:186775 ; ECO:0000269| PubMed:19965760 ; ECO:0000269| PubMed:20675723 ; ECO:0000269| PubMed:22731783
Active site 42 42 Proton acceptor (ATPase activity)
NUCLEOTIDE BINDING 102 103 ATP; 115 120 ATP; 335 337 ATP

Gene Ontology
Molecular Function GO ID Term Evidence
GO:0003677 F:DNA binding IDA
GO:0003918 F:DNA topoisomerase type II (ATP-hydrolyzing) activity IDA
GO:0005524 F:ATP binding IDA
GO:0008094 F:DNA-dependent ATPase activity IDA
GO:0034335 F:DNA supercoiling activity IDA
GO:0046872 F:metal ion binding IEA
Biological Process GO ID Term Evidence
GO:0006261 P:DNA-dependent DNA replication IEA
GO:0006265 P:DNA topological change IMP
GO:0006351 P:transcription, DNA-templated IMP
GO:0007059 P:chromosome segregation IBA
GO:0042493 P:response to drug IDA
GO:0046677 P:response to antibiotic IEA
Cellular Component GO ID Term Evidence
GO:0005694 C:chromosome IEA
GO:0005737 C:cytoplasm IDA
GO:0005829 C:cytosol IDA
GO:0009295 C:nucleoid IBA
GO:0009330 C:DNA topoisomerase complex (ATP-hydrolyzing) IDA
Mutation panel
Mutation Phenotype Reference
D426N nalidixic acid and enoxacin; nalidixic acid PMID:1656869
G164V nalidixic acid and enoxacin; nalidixic acid PMID:NA
K447E nalidixic acid and enoxacin; nalidixic acid PMID:1656869
R136C nalidixic acid and enoxacin; nalidixic acid PMID:1323022
R136E nalidixic acid and enoxacin; nalidixic acid PMID:1323022
R136G nalidixic acid and enoxacin; nalidixic acid PMID:1323022
R136H nalidixic acid and enoxacin; nalidixic acid PMID:1323022
R136I nalidixic acid and enoxacin; nalidixic acid PMID:1323022
R136L nalidixic acid and enoxacin; nalidixic acid PMID:1323022
R136S nalidixic acid and enoxacin; nalidixic acid PMID:1323022
W751R nalidixic acid and enoxacin; nalidixic acid PMID:1846808
Sequence
Multiple sequence alignment
MSA Mutation
Variant Position in MSA
D426N 433
G164V 168
K447E 454
R136C 140
R136E 140
R136G 140
R136H 140
R136I 140
R136L 140
R136S 140
W751R 762