gyrA - ARO:3003295-CCP42728.1
Structure
Entry features
| GENE | gyrA | |
|---|---|---|
| DESCRIPTION | Type IIA topoisomerase subunit GyrA | |
| ORGANISM | Mycobacterium tuberculosis H37Rv | |
| TAXONOMY | Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;Mycobacterium; Mycobacterium tuberculosis complex. | |
| UNIPROT | P9WG47 | |
| NCBI | CCP42728.1 | |
| ARO term | ARO:3003295 | |
| RESISTANCE | ciprofloxacin; enoxacin; fluoroquinolone; gatifloxacin; grepafloxacin; levofloxacin; lomefloxacin; moxifloxacin; nalidixic acid; norfloxacin; nybomycin; ofloxacin; pefloxacin; sparfloxacin; trovafloxacin |
Cross-references
| STRING | 83332.Rv0006 | |
|---|---|---|
| REFSEQ | NP_214520.1; NC_000962.3; WP_003917265.1; NZ_KK339370.1 | |
| PFAM | PF00521 - DNA_topoisoIV; PF03989 - DNA_gyraseA_C | |
| KEGG | mtu:Rv0006 | |
| EMBL | AL123456; CCP42728.1; JX303241; AFR90330.1; L11919; AAC36878.1; L27512; AAA83017.1; X72872; CAA51386.1 | |
| EC | 5.99.1.3 | |
| CHEMBL | CHEMBL4165 | |
| Catalytic activity | ATP-dependent breakage, passage and rejoining of double-stranded DNA; ECO:0000255| HAMAP-Rule:MF_01897 ; ECO:0000269| PubMed:15047530 ; ECO:0000269| PubMed:16876125 | |
| Active site | 129 129 O-(5'-phospho-DNA)-tyrosine intermediate |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0000287 | F:magnesium ion binding | IDA | |
| GO:0003677 | F:DNA binding | IEA | |
| GO:0003918 | F:DNA topoisomerase type II (ATP-hydrolyzing) activity | IDA | |
| GO:0005524 | F:ATP binding | IDA | |
| GO:0016887 | F:ATPase activity | IDA | |
| GO:0034335 | F:DNA supercoiling activity | IDA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0006261 | P:DNA-dependent DNA replication | IEA | |
| GO:0006265 | P:DNA topological change | IDA | |
| GO:0046677 | P:response to antibiotic | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005618 | C:cell wall | IDA | |
| GO:0005694 | C:chromosome | IEA | |
| GO:0005737 | C:cytoplasm | IEA | |
| GO:0005886 | C:plasma membrane | IDA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| A74S | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:17035499 |
| A90G | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| A90V | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 PMID:21300839 |
| A90V,D94G | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:16377674 |
| D89G | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| D89N | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| D89V | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| D94A | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 PMID:21300839 |
| D94G | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 PMID:21300839 |
| D94H | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 |
| D94N | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 PMID:21300839 |
| D94T | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:27847014 |
| D94V | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| D94Y | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 PMID:21300839 |
| G88A | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| G88C | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:21300839 |
| L109V | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:17434825 |
| P102H | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:16584301 |
| S91P | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:8031045 PMID:21300839 |
| S95T | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:17434825 |
| T80A | ciprofloxacin; fluoroquinolone; ofloxacin | PMID:16377674 PMID:17035499 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| A74S | 103 |
| A90G | 119 |
| A90V | 119 |
| A90V;D94G | 119;123 |
| D89G | 118 |
| D89N | 118 |
| D89V | 118 |
| D94A | 123 |
| D94G | 123 |
| D94H | 123 |
| D94N | 123 |
| D94T | 123 |
| D94V | 123 |
| D94Y | 123 |
| G88A | 117 |
| G88C | 117 |
| L109V | 138 |
| P102H | 131 |
| S91P | 120 |
| S95T | 124 |
| T80A | 109 |