gyrA - ARO:3003294-AAC75291.1
Structure
Entry features
| GENE | gyrA |
|---|---|
| ORGANISM | Escherichia coli str. K-12 substr. MG1655 |
| TAXONOMY | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;Enterobacteriaceae; Escherichia. |
| UNIPROT | P0AES4 |
| NCBI | AAC75291.1 |
| ARO term | ARO:3003294 |
| RESISTANCE | ciprofloxacin; enoxacin; fluoroquinolone; gatifloxacin; grepafloxacin; levofloxacin; lomefloxacin; moxifloxacin; nalidixic acid; norfloxacin; nybomycin; ofloxacin; pefloxacin; sparfloxacin; triclosan; trovafloxacin; quinolone |
Cross-references
| STRING | 316385.ECDH10B_2390 | |
|---|---|---|
| REFSEQ | NP_416734.1; NC_000913.3; WP_001281242.1; NZ_LN832404.1 | |
| PFAM | PF00521 - DNA_topoisoIV; PF03989 - DNA_gyraseA_C | |
| PATRIC | fig|1411691.4.peg.4 | |
| KEGG | ecj:JW2225; eco:b2231 | |
| EMBL | AP009048; BAA16048.1; M15631; AAA23948.1; U00096; AAC75291.1; X06373; CAA29676.1; X06744; CAA29919.1; Y00544; CAA68611.1 | |
| BioCyc/EcoCyc/MetaCyc | EcoCyc:EG10423-MONOMER; MetaCyc:EG10423-MONOMER | |
| INTACT | P0AES4 | |
| EC | 5.99.1.3 | |
| Drugbank | DB00537 - Ciprofloxacin | |
| CHEMBL | CHEMBL1858 | |
| Catalytic activity | ATP-dependent breakage, passage and rejoining of double-stranded DNA; ECO:0000255| HAMAP-Rule:MF_01897 ; ECO:0000269| PubMed:12051842 ; ECO:0000269| PubMed:18642932 ; ECO:0000269| PubMed:186775 ; ECO:0000269| PubMed:19965760 ; ECO:0000269| PubMed:9278055 | |
| Active site | 122 122 O-(5'-phospho-DNA)-tyrosine intermediate | |
| MINT | P0AES4 |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0003677 | F:DNA binding | IDA | |
| GO:0003918 | F:DNA topoisomerase type II (ATP-hydrolyzing) activity | IDA | |
| GO:0005524 | F:ATP binding | IEA | |
| GO:0008094 | F:DNA-dependent ATPase activity | IDA | |
| GO:0034335 | F:DNA supercoiling activity | IDA | |
| GO:0042802 | F:identical protein binding | IPI | |
| Biological Process | GO ID | Term | Evidence |
| GO:0006261 | P:DNA-dependent DNA replication | IEA | |
| GO:0006265 | P:DNA topological change | IMP | |
| GO:0006351 | P:transcription, DNA-templated | IDA | |
| GO:0042493 | P:response to drug | IMP | |
| GO:0046677 | P:response to antibiotic | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0005694 | C:chromosome | IEA | |
| GO:0005737 | C:cytoplasm | IDA | |
| GO:0005829 | C:cytosol | IDA | |
| GO:0016020 | C:membrane | HDA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| A67S | quinolone | PMID:2168148 PMID:2548439 |
| A84P | quinolone | PMID:2168148 |
| D87G | quinolone | PMID:25631675 |
| D87H | quinolone | PMID:25631675 |
| D87N | quinolone | PMID:2168148, PMID:2834621 PMID:25631675 |
| D87V | quinolone | PMID:1850972 |
| D87Y | quinolone | PMID:25631675 |
| G81C | quinolone | PMID:2168148 |
| Q106H | quinolone | PMID:2168148 PMID:2548439 |
| Q106R | quinolone | PMID:NA |
| S83A | quinolone | PMID:NA |
| S83I | quinolone | PMID:25631675 |
| S83L | quinolone | PMID:1850972, PMID:2168148 PMID:25631675 PMID:8388197 |
| S83L,D87N | quinolone | PMID:7840592 |
| S83L,D87Y | quinolone | PMID:7840592 |
| S83W | quinolone | PMID:1850972, PMID:2168148, PMID:2548439 PMID:1850972 |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| A67S | 68 |
| A84P | 85 |
| D87G | 88 |
| D87H | 88 |
| D87N | 88 |
| D87V | 88 |
| D87Y | 88 |
| G81C | 82 |
| Q106H | 107 |
| Q106R | 107 |
| S83A | 84 |
| S83I | 84 |
| S83L | 84 |
| S83L;D87N | 84;88 |
| S83L;D87Y | 84;88 |
| S83W | 84 |