rpoB - ARO:3003283-CCP43410.1
Structure
Entry features
| GENE | rpoB | |
|---|---|---|
| DESCRIPTION | RNA polymerase subunit beta | |
| ORGANISM | Mycobacterium tuberculosis H37Rv | |
| TAXONOMY | Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;Mycobacterium; Mycobacterium tuberculosis complex. | |
| UNIPROT | P9WGY9 | |
| NCBI | CCP43410.1 | |
| ARO term | ARO:3003283 | |
| RESISTANCE | peptide; rifabutin; rifampin; rifamycin; rifapentine; rifaximin; rifampicin |
Cross-references
| STRING | 83332.Rv0667 | |
|---|---|---|
| REFSEQ | NP_215181.1; NC_000962.3 | |
| PFAM | PF00562 - RNA_pol_Rpb2_6; PF04560 - RNA_pol_Rpb2_7; PF04561 - RNA_pol_Rpb2_2; PF04563 - RNA_pol_Rpb2_1; PF04565 - RNA_pol_Rpb2_3; PF10385 - RNA_pol_Rpb2_45 | |
| PATRIC | fig|83332.12.peg.742 | |
| KEGG | mtu:Rv0667 | |
| EMBL | AF147033; AAD45507.1; AF147030; AAD45505.1; AL123456; CCP43410.1; ALT_INIT; L05910; AAB59068.1; U12205; AAA20242.2; AF147031; AAD45506.1; AF143771; AAD29720.1; S71246; AAB31207.1; AF146567; AAD37379.1; AF147034; AAD45508.1; L27989; AAA21416.1 | |
| INTACT | P9WGY9 | |
| EC | 2.7.7.6 | |
| CHEMBL | CHEMBL2006 | |
| Catalytic activity | Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1); ECO:0000255| HAMAP-Rule:MF_01321 |
Gene Ontology
| Molecular Function | GO ID | Term | Evidence |
|---|---|---|---|
| GO:0003677 | F:DNA binding | IEA | |
| GO:0003899 | F:DNA-directed 5'-3' RNA polymerase activity | IEA | |
| GO:0032549 | F:ribonucleoside binding | IEA | |
| Biological Process | GO ID | Term | Evidence |
| GO:0006351 | P:transcription, DNA-templated | IEA | |
| GO:0046677 | P:response to antibiotic | IEA | |
| Cellular Component | GO ID | Term | Evidence |
| GO:0000428 | C:DNA-directed RNA polymerase complex | IBA | |
| GO:0005618 | C:cell wall | IDA | |
| GO:0005829 | C:cytosol | IDA | |
| GO:0005886 | C:plasma membrane | IDA |
Mutation panel
| Mutation | Phenotype | Reference |
|---|---|---|
| L571V | resistant | PMID:11136757 |
| S531L | resistant | PMID:25427352 PMID:21300839 PMID:22708343 |
| H526C | resistant | PMID:25427352 |
| L533R | resistant | PMID:25427352 |
| L538R | resistant | PMID:25427352 |
| H526Y | resistant | PMID:21300839 PMID:22708343 PMID:15047531 |
| D516V | resistant | PMID:21300839 PMID:22708343 |
| L533P | resistant | PMID:22708343 |
| S531W | resistant | PMID:22708343 PMID:15047531 |
| S531G | resistant | PMID:22708343 |
| H526D | resistant | PMID:22708343 |
| H526S | resistant | PMID:22708343 |
| K527Q | resistant | PMID:22708343 |
| D516T | resistant | PMID:22708343 |
| D516Y | resistant | PMID:22708343 |
| M515I | resistant | PMID:22708343 |
| M515V | resistant | PMID:22708343 |
| L511P | resistant | PMID:22708343 PMID:10423225 |
| I572F | resistant | PMID:22708343 PMID:15814606 |
| S522L | resistant | PMID:15047531 PMID:18294243 |
| V176F | resistant | PMID:11136757 PMID:15728936 |
| Q438K | resistant | PMID:11136757 |
| D441V | resistant | PMID:11136757 |
| D441Y | resistant | PMID:11136757 |
| S447Q | resistant | PMID:11136757 |
| H451Y | resistant | PMID:11136757 |
| H451C | resistant | PMID:11136757 |
| H451D | resistant | PMID:11136757 |
| H451R | resistant | PMID:11136757 |
| S456L | resistant | PMID:11136757 |
| S456W | resistant | PMID:11136757 |
| V146F | resistant | PMID:10722516 |
| A381V | resistant | PMID:8870258 |
| L521P | resistant | PMID:8870258 |
| H526R | resistant | PMID:12454132 PMID:10423225 |
| S522W | resistant | PMID:12454132 |
| Q513P | resistant | PMID:12454132 |
| Q490H | resistant | PMID:12454132 |
| E504A | resistant | PMID:10423225 |
| Q513L | resistant | PMID:10423225 |
| Q513K | resistant | PMID:10423225 |
| D516N | resistant | PMID:10423225 |
| D516G | resistant | PMID:10423225 |
| H526L | resistant | PMID:10423225 |
| S450L | resistant | PMID:17108078 |
| S509R | resistant | PMID:17108078 |
| S509Q | resistant | PMID:19547874 |
| Q517L | resistant | PMID:19547874 |
| L524S | resistant | PMID:19547874 |
| R528P | resistant | PMID:19547874 |
| H526T | resistant | PMID:15184414 |
| R529Q | resistant | PMID:15184414 |
| H526N | resistant | PMID:15184414 |
| S512I | resistant | PMID:15184414 |
| L511R | resistant | PMID:8027320 |
| S512T | resistant | PMID:8027320 |
| S531C | resistant | PMID:8027320 |
| H526E | resistant | PMID:8027320 |
| L511V | resistant | PMID:11796356 |
| N518T | resistant | PMID:11796356 |
| S522Q | resistant | PMID:11796356 |
| K527N | resistant | PMID:11796356 |
| R528H | resistant | PMID:11796356 |
| S512R | resistant | PMID:10645439 |
| L521M | resistant | PMID:10645439 |
| H526G | resistant | PMID:10645439 |
| F505L | resistant | PMID:17360809 |
| S574L | resistant | PMID:17360809 |
| G507S | resistant | PMID:19721079 |
| G507D | resistant | PMID:19721079 |
| T508P | resistant | PMID:19721079 |
| T508A | resistant | PMID:19721079 |
| T508H | resistant | PMID:19721079 |
| Q513D | resistant | PMID:19721079 |
| Q513E | resistant | PMID:19721079 |
| D516H | resistant | PMID:19721079 |
| N519K | resistant | PMID:19721079 |
| G523A | resistant | PMID:19721079 |
| H526F | resistant | PMID:19721079 |
| H526Q | resistant | PMID:19721079 |
| S531F | resistant | PMID:19721079 |
| S531L,S622A | resistant | PMID:18294243 |
| H526S,M515V | resistant | PMID:18294243 |
| H526S,P535H | resistant | PMID:18294243 |
| H526Y,E541G | resistant | PMID:18294243 |
| D516Y,L511R | resistant | PMID:18294243 |
| D516E,S522L | resistant | PMID:18294243 |
| D518H | resistant | PMID:9003625 |
| S531L,F514V | resistant | PMID:10921994 |
| S531L,H526C | resistant | PMID:10921994 |
| L511R,D516V | resistant | PMID:10921994 |
| L511P,M515I | resistant | PMID:10921994 |
| L524W,T525P,H526Q,-527K | resistant | PMID:PMID in progress (multiple) |
| Q513H,-514F,-515M,-516D | resistant | PMID:PMID in progress (multiple) |
| Q513R | resistant | PMID:15814606 |
| H526P | resistant | PMID:15814606 |
| Q517H | resistant | PMID:18600483 |
| T508S | resistant | PMID:11474030 |
| L511M | resistant | PMID:11474030 |
| D516K | resistant | PMID:11474030 |
| N518H | resistant | PMID:11474030 |
| N518I | resistant | PMID:15728936 |
| -519N,S522L,S531L | resistant | PMID:PMID in progress (multiple) |
| H526D,S531 | resistant | PMID:10074552 |
| L511P,S512T,D516V | resistant | PMID:10074552 |
| T516I,G523W,D525Y | resistant | PMID:10074552 |
| H526D,E541G,S553A | resistant | PMID:10074552 |
| H526P,K527Q | resistant | PMID:7840574 |
| L538P | resistant | PMID:17970586 |
| L545M | resistant | PMID:18653964 |
| E562G,P564L | resistant | PMID:19520715 |
| R633C | resistant | PMID:11136757 |
| E672D | resistant | PMID:11136757 |
| -515M,-516D,-517Q,-518N | resistant | PMID:PMID in progress (multiple) |
| T508N | resistant | PMID:28904673 |
| S512N | resistant | PMID:28904673 |
| P520T | resistant | PMID:28904673 |
| V423A | resistant | PMID:NA |
| L436P | resistant | PMID:NA |
| S437T | resistant | PMID:NA |
| Q438L | resistant | PMID:NA |
| F439V | resistant | PMID:NA |
| H451L | resistant | PMID:NA |
| H451N | resistant | PMID:NA |
| H451P | resistant | PMID:NA |
| H451Q | resistant | PMID:NA |
| S456Q | resistant | PMID:NA |
| L458P | resistant | PMID:NA |
Sequence
Multiple sequence alignment
MSA Mutation
| Variant | Position in MSA |
|---|---|
| L571V | 580 |
| S531L | 540 |
| H526C | 535 |
| L533R | 542 |
| L538R | 547 |
| H526Y | 535 |
| D516V | 525 |
| L533P | 542 |
| S531W | 540 |
| S531G | 540 |
| H526D | 535 |
| H526S | 535 |
| K527Q | 536 |
| D516T | 525 |
| D516Y | 525 |
| M515I | 524 |
| M515V | 524 |
| L511P | 520 |
| I572F | 581 |
| S522L | 531 |
| V176F | 182 |
| Q438K | 447 |
| D441V | 450 |
| D441Y | 450 |
| S447Q | 456 |
| H451Y | 460 |
| H451C | 460 |
| H451D | 460 |
| H451R | 460 |
| S456L | 465 |
| S456W | 465 |
| V146F | 152 |
| A381V | 390 |
| L521P | 530 |
| H526R | 535 |
| S522W | 531 |
| Q513P | 522 |
| Q490H | 499 |
| E504A | 513 |
| Q513L | 522 |
| Q513K | 522 |
| D516N | 525 |
| D516G | 525 |
| H526L | 535 |
| S450L | 459 |
| S509R | 518 |
| S509Q | 518 |
| Q517L | 526 |
| L524S | 533 |
| R528P | 537 |
| H526T | 535 |
| R529Q | 538 |
| H526N | 535 |
| S512I | 521 |
| L511R | 520 |
| S512T | 521 |
| S531C | 540 |
| H526E | 535 |
| L511V | 520 |
| N518T | 527 |
| S522Q | 531 |
| K527N | 536 |
| R528H | 537 |
| S512R | 521 |
| L521M | 530 |
| H526G | 535 |
| F505L | 514 |
| S574L | 583 |
| G507S | 516 |
| G507D | 516 |
| T508P | 517 |
| T508A | 517 |
| T508H | 517 |
| Q513D | 522 |
| Q513E | 522 |
| D516H | 525 |
| N519K | 528 |
| G523A | 532 |
| H526F | 535 |
| H526Q | 535 |
| S531F | 540 |
| S531L;S622A | 540;631 |
| H526S;M515V | 535;524 |
| H526S;P535H | 535;544 |
| H526Y;E541G | 535;550 |
| D516Y;L511R | 525;520 |
| D516E;S522L | 525;531 |
| D518H | 527 |
| S531L;F514V | 540;523 |
| S531L;H526C | 540;535 |
| L511R;D516V | 520;525 |
| L511P;M515I | 520;524 |
| L524W;T525P;H526Q;-527K | 533;534;535 |
| Q513H;-514F;-515M;-516D | 522 |
| Q513R | 522 |
| H526P | 535 |
| Q517H | 526 |
| T508S | 517 |
| L511M | 520 |
| D516K | 525 |
| N518H | 527 |
| N518I | 527 |
| -519N;S522L;S531L | 531;540 |
| H526D;S531 | 535;59 |
| L511P;S512T;D516V | 520;521;525 |
| T516I;G523W;D525Y | 525;532;534 |
| H526D;E541G;S553A | 535;550;562 |
| H526P;K527Q | 535;536 |
| L538P | 547 |
| L545M | 554 |
| E562G;P564L | 571;573 |
| R633C | 642 |
| E672D | 681 |
| T508N | 517 |
| S512N | 521 |
| P520T | 529 |
| V423A | 432 |
| L436P | 445 |
| S437T | 446 |
| Q438L | 447 |
| F439V | 448 |
| H451L | 460 |
| H451N | 460 |
| H451P | 460 |
| H451Q | 460 |
| S456Q | 465 |
| L458P | 467 |